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PLANT TISSUE CULTURE
Related to country: Kenya

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Hi guys.
Recently I started this Internship at the University I go to, on plant tissue culture and I must say, I have found the love of my life!
Plant tissue culture is the in Vitro Micro propagation of Plants with the aim of either producing many Uniform plants at a go, or, transforming the plants Using Vectors such as plasmids or Bacteriophage chromosomes.
Explants used in tissue culture include stem cuttings, leaves, petioles, etc. These explants are then planted on nutrient media, and the planting is done in sterile environment, e.g., using a microflow.
The plants are then manipulated using different balances of hormones, the 2 main types of hormones used being Auxins e.g., NAA, IBA and 2, 4-D, and Cytokinines like BAP and TDZ.
For most Dicotyledonous plants, a high Auxins: Cytokinines ratio induces rooting. I have not worked on many Monocotyledonous plants but it is expected that the same ratio will induce shoot development in these plants.
These ratios have to be experimentally determined because optimum concentrations differ for the different plants.
Infact I would say that for me, this is the thrill for me. Experimentally determining these ratios and watching the different plants react to them.
After the plant has developed fully, we rinse the media off especially at the root area, then rise the roots with a fungicide to prevent infection by fungus. Simple rinsing it with water will lead to dumping off.
Then, we take them to a nursery for them to harden, after which they are taken to the green house.
It feels really nice to see that one leaf generated 100 plants which you are now taking to the green house and for them to be sold. Earning money doing what you love doing!!!!
Right now we are working on the common bean, Jatropha curcas, Melia volkensii, Lycopersicon esculentum and Saintpaulia ionantha.

August 12, 2008 | 12:08 PM Comments  1 comments

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NELIMA FIONA MUMOKI
August 12, 2008 | 12:49 PM
this is coool
gys this is a true must read!
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